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Beilstein J. Org. Chem. 2010, 6, 922–931, doi:10.3762/bjoc.6.98
Graphical Abstract
Scheme 1: Synthesis of magnetic biocatalyst particles.
Figure 1: Structures of comonomers employed in the synthesis of functional core–shell particles.
Figure 2: TEM images of a) Fe3O4 nanoparticles electrostatically stabilized by citric acid; b) Fe3O4@P(M100) ...
Figure 3: a) Cloud point temperature Tc of Fe3O4@P(OxMy) in water in relation to molar fraction of MEMA χM,ex...
Figure 4: a) Normalized magnetization loops of dispersions based on Fe3O4@CA in water (solid line), Fe3O4@CPS...
Scheme 2: Reaction scheme of the enzymatic digestion of BAPNA catalyzed by magnetically labeled trypsin ().
Figure 5: a) Cornish-Bowden diagram for the temperature-dependent kinetic data of trypsin activity, and b) Ea...
Scheme 3: Proposed mechanism of catalytic activity after heating magnetic biocatalyst particles above Tc.
Figure 6: a) Turnover number kcat and b) Michaelis constant Km of particle- immobilized trypsin vs free tryps...